研究領域細胞生物 免疫學 信號轉(zhuǎn)導
抗體來源Rabbit
克隆類型Polyclonal
交叉反應Human, Mouse, Rat, (predicted: Dog, Cow, Horse, )
產(chǎn)品應用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量64kDa
細胞定位細胞漿 細胞膜
性 狀Liquid
濃 度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human Nox-4: 81-180/578 <Cytoplasmic>
亞 型IgG
純化方法affinity purified by Protein A
緩 沖 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMedPubMed
產(chǎn)品介紹Nox4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of angiotensin II increases Nox4 expression, which suggests a role for Nox-4 in vascular oxidative stress response.
Function:
Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB.
Subunit:
Interacts with, relocalizes and stabilizes CYBA/p22phox. Interacts with TLR4. Interacts with protein disulfide isomerase.
Subcellular Location:
Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction, focal adhesion. Cell membrane. Note=May localize to plasma membrane and focal adhesions.
Tissue Specificity:
EXpressed in brain, in all layers of the cerebellum, in pyramidal cells of the Ammon horn and in Purkinje cells (at protein level). Expressed in osteoclasts, leukocytes, kidney, liver and lung.
Similarity:
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain.
SWISS:
Q9JHI8
Gene ID:
50507
