β-肌動蛋白/β-Actin（內參）抗體
- 發布日期： 2023-11-08
- 更新日期： 2025-10-10
產品詳請
| 產地 |
上海
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| 品牌 |
森興研
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| 保存條件 |
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
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| 貨號 |
|
| 用途 |
|
| 應用范圍 |
WB=1:5000-50000 ELISA=1:5000-20000 IHC-P=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 （石蠟切片需做抗原修復） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
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| 抗原來源 |
Rabbit
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| 保質期 |
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| 抗體名 |
beta-Actin (Loading Control)
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| 是否單克隆 |
否
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| 克隆性 |
是
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| 靶點 |
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| 適應物種 |
Human, Mouse, Rat, Hamster, 3 (predicted: Chicken, Dog, Pig, Rabbit, Sheep, Bee, Fish, Guinea Pig, Cat, 2)
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| 形態 |
Liquid
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| 宿主 |
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| 標記物 |
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| 包裝規格 |
100ul
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| 亞型 |
IgG
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| 標識物 |
|
| 濃度 |
1mg/ml%
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| 免疫原 |
Synthetic MAP peptide derived from human beta-Actin: 1-200/375
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| 是否進口 |
否
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產品類型內參抗體
研究領域腫瘤 細胞生物 信號轉導 細胞骨架
抗體來源Rabbit
克隆類型Polyclonal
交叉反應Human, Mouse, Rat, Hamster, 3 (predicted: Chicken, Dog, Pig, Rabbit, Sheep, Bee, Fish, Guinea Pig, Cat, 2)
產品應用WB=1:5000-50000 ELISA=1:5000-20000 IHC-P=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量42kDa
細胞定位細胞漿
性 狀Liquid
濃 度1mg/ml
免 疫 原Synthetic MAP peptide derived from human beta-Actin: 1-200/375
亞 型IgG
純化方法affinity purified by Protein A
緩 沖 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMedPubMed
產品介紹Loading Control
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008].
Function:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subunit:
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2.
Subcellular Location:
Cytoplasm. cytoskeleton.
Tissue Specificity:
Ubiquitously expressed in all eukaryotic cells.
Post-translational modifications:
ISGylated.
Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.
DISEASE:
Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Similarity:
Belongs to the actin family.
SWISS:
P60709
Gene ID:
60
